Haematological Changes in Male Albino Rats Administered Silver Nanoparticles
Ikwebe Joseph
Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, Nigeria.
Moses Adondua Abah *
Department of Biochemistry, Faculty of Pure and Applied Sciences, Federal University Wukari, Taraba State, Nigeria and Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Federal University Wukari, Taraba State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Silver Nanoparticles are by far the most studied in literature due to their wide and rapidly growing applications in a number of scientific areas and in consumer products. However their toxic effects on the environment and human health are yet to be fully investigated. Therefore, the present study aimed at investigating haematological changes on male albino rats administered with Silver Nanoparticles. Sixteen male albino rats were randomly assigned into four experimental groups 1- 4 of four rats per group. Group 1 served as the control and received potable water. Groups 2 to 4 were orally exposed to varying concentrations of Silver Nanoparticles (AgNPs) at 100, 250, and 500mg/kg daily for 14 days. Following cessation of treatments, rats were sacrificed and the blood were collected in heparinized bottles haematological studies. Th results revealed that rat exposure to AgNPs caused no significant difference (p>0.05) on the following haematological parameters: Red Blood Cells (RBC), Packed Cell Volume (PCV), Mean Corpuscular Volume (MCV), Mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Haemoglobin Concentration (MCHC), Haemoglobin Concentration (Hb), Neutrophils (NEU) and Mixed Cell Percentage (MXD) while WBC and LYM showed significant differences in some groups. At lower dosage of 100mg/kg and 250mg/kg which is slightly below the lethal dosage of AgNPs, rats showed no significantly difference in most of the groups. From the result obtained, it can be deduced, among all the haematological evaluated, RBC is higher in group 1 (5.18±0.25) and least at group 1 (5.18±0.25). PCV is higher in group 2 (44.17±7.30) and least in group 3 (36.03±1.38). MCH is higher in group 2 (31.83±0.71) and least in group 4 (26.93±4.40). MCV is higher in group 3 (66.75±18.74) and least in group 4 (52.81±5.93). MCHC is higher in group 4 (463±213.54) and least in group 4 (463±213.54). Hb is higher in group 2 (420.5±99.70) and least in group 4 (255±77.78). WBC is higher in group 1 (11.75±1.06) and least in group 3 (8.50±0.71). Neutrophils is higher in group 1 (75.34±4.87) and least in group 3 (54.00±5.66). Lymphocytes is higher in group 3 (39.00±1.41) and least in group 1 (16.29±1.36). MXD is higher in group 4 (14.89±4.77) and least in group 3 (7.00±4.24). All the values were within the normal range except WBC and Lymphocytes which showed significant difference p>0.05). It can be concluded that AgNPs administrated at lower dosage showed no significant difference on the haematological parameters of male albino rats.
Keywords: Silver nanoparticles, haematological parameters, albino rats, dosage, green synthesis, nanotechnology